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X-WR-CALNAME;VALUE=TEXT:HSCI Technology Series:  "All-optical electrophysiology"
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SUMMARY:HSCI Technology Series:  "All-optical electrophysiology"
DESCRIPTION:<p><strong>Guest presenters:</strong><br><em><strong>Adam Cohen, PhD</strong></em><br>Professor and Howard Hughes Medical Institute Investigator<br>Departments of Chemistry and Chemical Biology and of Physics<br>and</p><p><em><strong>Daniel Hochbaum, PhD</strong></em><br>Postdoctoral Fellow<br>Cohen Lab<br>Department of Chemistry and Chemical Biology</p><p><span style="color: #68a7bb;"><strong>Abstract:</strong></span><br>We recently developed a technology for probing the electrophysiology of cells using light-alone.  A blue light-activated ion channel triggers membrane depolarizations, while a red light-activated fluorescent voltage indicator protein reports the response.  We developed instrumentation for delivering arbitrarily complex patterns of blue light stimulation and for imaging the response with high resolution in space and time, over a large field of view.  We will discuss recent applications of "Optopatch" across many cell types: primary DRG and central neurons, hiPSC-derived neurons and cardiomyocytes, and several cell-based disease models.  A Cre-dependent transgenic mouse enables expression of the Optopatch genes in any tissue.  We will discuss the technical limitations and the infrastructure needed to use the technology. </p>
LOCATION:Harvard University, Sherman-Fairchild 261, 7 Divinity Ave., Cambridge, MA
STATUS:CONFIRMED
DTSTART:20141113T170000Z
DTEND:20141113T180000Z
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