#  Boston Single-Cell Network Meeting 

 



####  calendar\_today Date and Time 

 **April 1, 2014** 

 04:30PM - 06:00PM EDT 

####  pin\_drop Location 

 **Harvard Medical School, New Research Building, Room 350, 77 Avenue Louis Pasteur, Boston, MA**  



 

 



 

**Agenda:**  
***David Cacchiarelli*** (HFSP Fellow, Broad Institute)  
"Role of coding and non-coding RNAs in muscle differentiation and disease"

***Cole Trapnell*** (Post Doctoral Fellow, J. Rinn Lab)  
"The dynamics of cell fate decisions are revealed by pseudo-temporal ordering of single cells"  
Abstract:  
*Defining the transcriptional dynamics of a temporal process such as cell differentiation is challenging owing to the high variability in gene expression between individual cells. Time-series gene expression analyses of bulk cells have difficulty distinguishing early and late phases of a transcriptional cascade or identifying rare sub-populations of cells, and single-cell proteomic methods rely on a priori knowledge of key distinguishing markers1. Here we describe Monocle, an unsupervised algorithm that increases the temporal resolution of transcriptome dynamics using single-cell RNA-Seq data collected at multiple time points. Applied to the differentiation of primary human myoblasts, Monocle revealed switch-like changes in expression of key regulatory factors, sequential waves of gene regulation, and expression of regulators that were not known to have a role in differentiation. We validated some of these predicted regulators in a loss-of function screen. Monocle can in principle be used to recover single-cell gene expression kinetics from a wide array of cellular processes, including differentiation, proliferation and oncogenic transformation.*

Food and beverage will be provided at the meeting.  
  
Faculty organizers: Peter Kharchenko and Lev Silberstein



 

 



 

 

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