Program Leader: Andrew P. McMahon, PhD

The mission of the Genome Modification Facility is to offer to the community of scientists within the Harvard Stem Cell Institute a high level of expertise in mouse genetic engineering and the tools that permit the efficient generation of animal models for stem cell research in a cost-effective and timely fashion.  

• Production of transgenic mice by pronuclear microinjection of DNA constructs in plasmids, BACs or YACs
• Chimera construction by injection of ES cells into blastocysts or aggregations with morulae
• Cryopreservation and storage of embryos and sperm
• Mouse strain resuscitation by in vitro fertilization and intracytoplasmic sperm injection (ICSI)
• Re-derivation of mouse strains by embryo transfer
• Gene targeting in embryonic stem (ES) cells
• The development of lentiviral vector based transgenes
• Validation of DNA vectors, ES cells and feeder lines
• Consultation on genetic model creation

For questions and information, please visit the facility's website or contact:

Manfred Baetscher, Ph.D.
Director, Genome Modification Facility
16 Divinity Ave, Room 1062
Cambridge, MA 02138
(617) 495-7991
mbaetscher@mcb.harvard.edu