Research Newsletter - October 2009
Spotlight
In an article paper published in Stem Cells, HSCI Principal Faculty member Ole Isacson, MD, and colleagues report the identification of a combination of surface biomarkers that define neural lineage development of stem cells.
CD15, CD24 and CD29 Define a Surface Biomarker Code for Neural Lineage Differentiation of Stem Cells
Abstract:
Identification and use of cell surface cluster of differentiation (CD) biomarkers have enabled much scientific and clinical progress. We identify a CD surface antigen code for the neural linage based on combinatorial flow cytometric analysis of three distinct populations derived from human embryonic stem cells: (1) CD15(+)/CD29(HI)/CD24(LO) surface antigen expression defined neural stem cells. (2) CD15(--)/CD29(HI)/CD24(LO) revealed neural crest-like and mesenchymal phenotypes. (3) CD15(--)/CD29(LO)/CD24(HI) selected neuroblasts and neurons. Fluorescence-activated cell sorting (FACS) for the CD15(--)/CD29(LO)/CD24(HI) profile reduced proliferative cell types in human embryonic stem cell differentiation. This eliminated tumor formation in vivo, resulting in pure neuronal grafts. In conclusion, combinatorial CD15/CD24/CD29 marker profiles define neural lineage development of neural stem cell, neural crest and neuronal populations from human stem cells. We believe this set of biomarkers enables analysis and selection of neural cell types for developmental studies, pharmacological and therapeutic applications.
Pruszak J, Ludwig W, Blak A, Alavian K, Isacson O. CD15, CD24 and CD29 Define a Surface Biomarker Code for Neural Lineage Differentiation of Stem Cells. Stem Cells. 2009 Sep 1. [Epub ahead of print] Read Paper.
Review and Commentary
Borowiak M, Melton DA. How to make beta cells? Curr Opin Cell Biol. 2009 Sep 23. [Epub ahead of print]
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Chen AT, Zon LI. Zebrafish blood stem cells. J Cell Biochem. 2009 Sep 1;108(1):35-42.
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Humphreys BD, Atkins MB. Rapid Development of Hypertension by Sorafenib: Toxicity or Target? Clin Cancer Res. 2009 Sep 29. [Epub ahead of print]
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Latremoliere A, Woolf CJ. Central sensitization: a generator of pain hypersensitivity by central neural plasticity. J Pain. 2009 Sep;10(9):895-926.
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Maherali N, Hochedlinger K. Tgfbeta Signal Inhibition Cooperates in the Induction of iPSCs and Replaces Sox2 and cMyc. Curr Biol. 2009 Sep 16. [Epub ahead of print]
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Nathan DG, Orkin SH. Musings on genome medicine: the Obama effect redux. Genome Med. 2009 Sep 11;1(9):86. [Epub ahead of print]
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Pu WT. Identification of a cardiac disease modifier gene using forward genetics in the mouse. PLoS Genet. 2009 Sep;5(9):e1000643. Epub 2009 Sep 18.
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Schier AF, Needleman D. Developmental biology: Rise of the source-sink model. Nature. 2009 Sep 24;461(7263):480-1.
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Waikar SS, Betensky RA, Bonventre JV. Creatinine as the gold standard for kidney injury biomarker studies? Nephrol Dial Transplant. 2009 Sep 7. [Epub ahead of print]
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Williams DA. National Institutes of Health releases new guidelines on human stem cell research. Mol Ther. 2009 Sep;17(9):1485-6.
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Zhao W, Karp JM. Controlling cell fate in vivo. Chembiochem. 2009 Sep 21;10(14):2308-10.
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Blood
Amigo JD, Ackermann GE, Cope JJ, Yu M, Cooney JD, Ma D, Langer NB, Shafizadeh E, Shaw GC, Horsley W, Trede NS, Davidson AJ, Barut BA, Zhou Y, Wojiski SA, Traver D, Moran TB, Kourkoulis G, Hsu K, Kanki JP, Shah DI, Lin HF, Handin RI, Cantor AB, Paw BH. The role and regulation of friend of GATA-1 (FOG-1) during blood development in the zebrafish. Blood. 2009 Sep 3. [Epub ahead of print]
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Kotani A, Ha D, Hsieh J, Rao PK, Schotte D, den Boer ML, Armstrong SA, Lodish HF. miR-128b is a potent glucocorticoid sensitizer in MLL-AF4 acute lymphocytic leukemia cells and exerts cooperative effects with miR-221. Blood. 2009 Sep 11. [Epub ahead of print]
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Cancer
Ueno S, Tatetsu H, Hata H, Iino T, Niiro H, Akashi K, Tenen DG, Mitsuya H, Okuno Y. PU.1 induces apoptosis in myeloma cells through direct transactivation of TRAIL. Oncogene. 2009 Sep 14. [Epub ahead of print]
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Utikal J, Maherali N, Kulalert W, Hochedlinger K. Sox2 is dispensable for the reprogramming of melanocytes and melanoma cells into induced pluripotent stem cells. J Cell Sci. 2009 Oct 1;122(Pt 19):3502-10. Epub 2009 Sep 1.
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Wadlow RC, Wittner BS, Finley SA, Bergquist H, Upadhyay R, Finn S, Loda M, Mahmood U, Ramaswamy S. Systems-level modeling of cancer-fibroblast interaction. PLoS One. 2009 Sep 3;4(9):e6888.
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Cardiovascular System
Kusakari Y, Xiao CY, Himes N, Kinsella SD, Takahashi M, Rosenzweig A, Matsui T. Myocyte injury along myofibers in left ventricular remodeling after myocardial infarction. Interact Cardiovasc Thorac Surg. 2009 Sep 23. [Epub ahead of print]
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Sartoretto JL, Jin BY, Bauer M, Gertler FB, Liao R, Michel T. Regulation of VASP phosphorylation in cardiac myocytes: Differential regulation by cyclic nucleotides and modulation of protein expression in heart. Am J Physiol Heart Circ Physiol. 2009 Sep 4. [Epub ahead of print]
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Developmental Biology
Huang P, Schier AF. Dampened Hedgehog signaling but normal Wnt signaling in zebrafish without cilia. Development. 2009 Sep;136(18):3089-98.
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Papakostas TD, Pieretti-Vanmarcke R, Nicolaou F, Thanos A, Trichonas G, Koufomichali X, Anago K, Donahoe PK, Teixeira J, Maclaughlin DT, Vavvas D. Development of an efficiently cleaved, bioactive, highly pure FLAG-tagged recombinant human Mullerian Inhibiting Substance. Protein Expr Purif. 2009 Sep 13. [Epub ahead of print]
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Trimarchi JM, Cho SH, Cepko CL. Identification of genes expressed preferentially in the developing peripheral margin of the optic cup. Dev Dyn. 2009 Sep;238(9):2327-9.
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Genetics and Genomics
Yu F, Keinan A, Chen H, Ferland RJ, Hill RS, Mignault AA, Walsh CA, Reich D. Detecting natural selection by empirical comparison to random regions of the genome. Hum Mol Genet. 2009 Sep 25. [Epub ahead of print]
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Imaging
Vinegoni C, Razansky D, Hilderbrand SA, Shao F, Ntziachristos V, Weissleder R. Transillumination fluorescence imaging in mice using biocompatible upconverting nanoparticles. Opt Lett. 2009 Sep 1;34(17):2566-8. doi: 10.1364/OL.34.002566.
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Molecular Biology
Andersson KB, Finsen AV, Sjåland C, Winer LH, Sjaastad I, Odegaard A, Louch WE, Wang Y, Chen J, Chien KR, Sejersted OM, Christensen G. Mice carrying a conditional Serca2(flox) allele for the generation of Ca(2+) handling-deficient mouse models. Cell Calcium. 2009 Sep;46(3):219-25. Epub 2009 Aug 18.
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Muscular System
Morissette M, Cook S, Buranasombati C, Rosenberg M, Rosenzweig A. Myostatin Inhibits IGF-I Induced Myotube Hypertrophy Through Akt. Am J Physiol Cell Physiol. 2009 Sep 16. [Epub ahead of print]
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Nervous System
Poduri A, Chitsazzadeh V, D'Arrigo S, Fedrizzi E, Pantaleoni C, Riva D, Busse C, Küster H, Duplessis A, Gaitanis J, Sahin M, Garganta C, Topcu M, Dies KA, Barry BJ, Partlow J, Barkovich AJ, Walsh CA, Chang BS. The syndrome of perisylvian polymicrogyria with congenital arthrogryposis. Brain Dev. 2009 Sep 12. [Epub ahead of print]
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Pruszak J, Ludwig W, Blak A, Alavian K, Isacson O. CD15, CD24 and CD29 Define a Surface Biomarker Code for Neural Lineage Differentiation of Stem Cells. Stem Cells. 2009 Sep 1. [Epub ahead of print]
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Renal System
Liangos O, Tighiouart H, Perianayagam MC, Kolyada A, Han WK, Wald R, Bonventre JV, Jaber BL. Comparative analysis of urinary biomarkers for early detection of acute kidney injury following cardiopulmonary bypass. Biomarkers. 2009 Sep;14(6):423-31.
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Technology
Amit I, Garber M, Chevrier N, Leite AP, Donner Y, Eisenhaure T, Guttman M, Grenier JK, Li W, Zuk O, Schubert LA, Birditt B, Shay T, Goren A, Zhang X, Smith Z, Deering R, McDonald RC, Cabili M, Bernstein BE, Rinn JL, Meissner A, Root DE, Hacohen N, Regev A. Unbiased Reconstruction of a Mammalian Transcriptional Network Mediating Pathogen Responses. Science. 2009 Sep 3. [Epub ahead of print]
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Tissue Engineering
Hjortnaes J, Gottlieb D, Figueiredo JL, Melero-Martin J, Kohler RH, Bischoff J, Weissleder R, Mayer J, Aikawa E. Intravital Molecular Imaging of Small-Diameter Tissue-Engineered Vascular Grafts: A Feasibility Study. Tissue Eng Part C Methods. 2009 Sep 14. [Epub ahead of print]
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Weinand C, Gupta R, Weinberg E, Madisch I, Neville CM, Jupiter JB, Vacanti JP. Toward regenerating a human thumb in situ. Tissue Eng Part A. 2009 Sep;15(9):2605-15.
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