Research Newsletter - May 2009
Spotlight
In an article paper published in Cerebral Cortex, HSCI Executive Committee Member Jeffrey Macklis, MD, D.HST, and colleagues show that two neuronal subtypes initially share aspects of molecular identity that are progressively resolved during differentiation.
Lmo4 and Clim1 Progressively Delineate Cortical Projection Neuron Subtypes during Development
Abstract:
Molecular controls over the development of the exceptional neuronal subtype diversity of the cerebral cortex are now beginning to be identified. The initial subtype fate decision early in the life of a neuron, and the malleability of this fate when the balance of key postmitotic signals is modified, reveals not only that a neuron is deterministically set on a general developmental path at its birth, but also that this program must be precisely executed during postmitotic differentiation. Here, we show that callosal projection neurons (CPN) and subcerebral projection neurons (subcerebral PN) in layer V of the neocortex share aspects of molecular identity after their birth that are progressively resolved during differentiation. The LIM-homeodomain-related genes Lmo4 and Clim1 are initially expressed by both CPN and subcerebral PN in layer V, and only during mid to late differentiation does expression of Lmo4 and Clim1 become largely segregated into distinct neuronal subtypes. This progressive postmitotic resolution of molecular identity reveals similarities and possibly shared evolutionary origin between layer V CPN and subcerebral PN, and provides insight into how and when these neuronal subtypes achieve their distinct identities during cortical development.
Azim E, Shnider SJ, Cederquist GY, Sohur US, Macklis JD. Lmo4 and Clim1 Progressively Delineate Cortical Projection Neuron Subtypes during Development. Cereb Cortex. 2009 Apr 14. [Epub ahead of print] Read paper.
Review and Commentary
Bonventre JV. Microvesicles from mesenchymal stromal cells protect against acute kidney injury. J Am Soc Nephrol. 2009 May;20(5):927-8. Epub 2009 Apr 23.
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Cheung KJ, Ebert BL. Therapeutic potential of targeting haploinsufficient genes in cancer. Pediatr Blood Cancer. 2009 Apr 2. [Epub ahead of print] PubMed
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Clohessy JG, Pandolfi PP. beta-tting on p63 as a metastatic suppressor. Cell. 2009 Apr 3;137(1):28-30.
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Jaffer FA, Libby P, Weissleder R. Optical and Multimodality Molecular Imaging. Insights Into Atherosclerosis. Arterioscler Thromb Vasc Biol. 2009 Apr 9. [Epub ahead of print]
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Lane SW, Scadden DT, Gilliland DG. The leukemic stem cell niche - current concepts and therapeutic opportunities. Blood. 2009 Apr 28. [Epub ahead of print]
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Montgomery RK, Shivdasani RA. Prominin1 (CD133) as an Intestinal Stem Cell Marker: Promise and Nuance. Gastroenterology. 2009 Apr 28. [Epub ahead of print]
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Nathan DG, Orkin SH. Musings on genome medicine: gene therapy. Genome Med. 2009 Apr 3;1(4):38.
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Weir GC, Koulamnda M. Control of inflammation with alpha1-antitrypsin: a potential treatment for islet transplantation and new-onset type 1 diabetes. Curr Diab Rep. 2009 Apr;9(2):100-2.
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Williams DA. Progress in genetic therapy for severe combined immunodeficiency associated with adenosine deaminase deficiency. Mol Ther. 2009 Apr;17(4):577-8.
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Blood
Heinrichs S, Kulkarni RV, Bueso-Ramos CE, Levine RL, Loh ML, Li C, Neuberg D, Kornblau SM, Issa JP, Gilliland DG, Garcia-Manero G, Kantarjian HM, Estey EH, Look AT. Accurate detection of uniparental disomy and microdeletions by SNP array analysis in myelodysplastic syndromes with normal cytogenetics. Leukemia. 2009 Apr 23. [Epub ahead of print]
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Hoogenkamp M, Lichtinger M, Krysinska H, Lancrin C, Clarke D, Williamson A, Mazzarella L, Ingram R, Jorgensen H, Fisher A, Tenen DG, Kouskoff V, Lacaud G, Bonifer C. Early chromatin unfolding by RUNX1 - a molecular explanation for differential requirements during specification versus maintenance of the hematopoietic gene expression program. Blood. 2009 Apr 1. [Epub ahead of print]
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Cancer
Bullain SS, Sahin A, Szentirmai O, Sanchez C, Lin N, Baratta E, Waterman P, Weissleder R, Mulligan RC, Carter BS. Genetically engineered T cells to target EGFRvIII expressing glioblastoma. J Neurooncol. 2009 Apr 23. [Epub ahead of print]
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Carver BS, Tran J, Gopalan A, Chen Z, Shaikh S, Carracedo A, Alimonti A, Nardella C, Varmeh S, Scardino PT, Cordon-Cardo C, Gerald W, Pandolfi PP. Aberrant ERG expression cooperates with loss of PTEN to promote cancer progression in the prostate. Nat Genet. 2009 May;41(5):619-24. Epub 2009 Apr 26.
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Laane E, Tamm KP, Buentke E, Ito K, Khahariza P, Oscarsson J, Corcoran M, Björklund AC, Hultenby K, Lundin J, Heyman M, Säderhäll S, Mazur J, Porwit A, Pandolfi PP, Zhivotovsky B, Panaretakis T, Grandér D. Cell death induced by dexamethasone in lymphoid leukemia is mediated through initiation of autophagy. Cell Death Differ. 2009 Apr 24. [Epub ahead of print]
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Cardiovascular System
Jain M, Jakubowski A, Cui L, Shi J, Su L, Bauer M, Guan J, Lim CC, Naito Y, Thompson JS, Sam F, Ambrose C, Parr M, Crowell T, Lincecum JM, Wang MZ, Hsu YM, Zheng TS, Michaelson JS, Liao R, Burkly LC. A novel role for tumor necrosis factor-like weak inducer of apoptosis (TWEAK) in the development of cardiac dysfunction and failure. Circulation. 2009 Apr 21;119(15):2058-68. Epub 2009 Apr 6.
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Developmental Biology
Borowiak M, Maehr R, Chen S, Chen AE, Tang W, Fox JL, Schreiber SL, Melton DA. Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell. 2009 Apr 3;4(4):348-58.
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Dovey M, Patton EE, Bowman T, North T, Goessling W, Zhou Y, Zon LI. Topoisomerase II alpha is required for embryonic development and liver regeneration in zebrafish. Mol Cell Biol. 2009 Apr 20. [Epub ahead of print]
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Guseh JS, Bores SA, Stanger BZ, Zhou Q, Anderson WJ, Melton DA, Rajagopal J. Notch signaling promotes airway mucous metaplasia and inhibits alveolar development. Development. 2009 May;136(10):1751-9. Epub 2009 Apr 15.
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Müller MR, Sasaki Y, Stevanovic I, Lamperti ED, Ghosh S, Sharma S, Gelinas C, Rossi DJ, Pipkin ME, Rajewsky K, Hogan PG, Rao A. Requirement for balanced Ca/NFAT signaling in hematopoietic and embryonic development. Proc Natl Acad Sci USA. 2009 Apr 28;106(17):7034-9. Epub 2009 Apr 7.
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Mao J, McGlinn E, Huang P, Tabin CJ, McMahon AP. Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb. Dev Cell. 2009 Apr;16(4):600-6.
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Triboulet R, Chang HM, Lapierre RJ, Gregory RI. Post-transcriptional control of DGCR8 expression by the Microprocessor. RNA. 2009 Apr 21. [Epub ahead of print]
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Wuelling M, Kaiser FJ, Buelens LA, Braunholz D, Shivdasani RA, Depping R, Vortkamp A. Trps1, a regulator of chondrocyte proliferation and differentiation, interacts with the activator form of Gli3. Dev Biol. 2009 Apr 1;328(1):40-53. Epub 2009 Jan 14.
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Diabetes
Kondo T, El Khattabi I, Nishimura W, Laybutt DR, Geraldes P, Shah S, King G, Bonner-Weir S, Weir G, Sharma A. p38 MAPK is a major regulator of MafA protein stability under oxidative stress. Mol Endocrinol. 2009 Apr 30. [Epub ahead of print]
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Genetics and Genomics
Ali AM, Kirby M, Jansen M, Lach FP, Schulte J, Singh TR, Batish SD, Auerbach AD, Williams DA, Meetei AR. Identification and characterization of mutations in FANCL gene: A second case of Fanconi anemia belonging to FA-L complementation group. Hum Mutat. 2009 Apr 29. [Epub ahead of print]
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Arashiro P, Eisenberg I, Kho AT, Cerqueira AM, Canovas M, Silva HC, Pavanello RC, Verjovski-Almeida S, Kunkel LM, Zatz M. Transcriptional regulation differs in affected facioscapulohumeral muscular dystrophy patients compared to asymptomatic related carriers. Proc Natl Acad Sci USA. 2009 Apr 14;106(15):6220-5. Epub 2009 Apr 1.
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Hu G, Kim J, Xu Q, Leng Y, Orkin SH, Elledge SJ. A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal. Genes Dev. 2009 Apr 1;23(7):837-48.
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Imaging
Daniel KD, Kim GY, Vassiliou CC, Galindo M, Guimaraes AR, Weissleder R, Charest A, Langer R, Cima MJ. Implantable diagnostic device for cancer monitoring. Biosens Bioelectron. 2009 Apr 16. [Epub ahead of print]
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Immunology
Bianchi T, Pincus LB, Wurbel MA, Rich BE, Kupper TS, Fuhlbrigge RC, Boes M. Maintenance of peripheral tolerance through controlled tissue homing of antigen-specific T cells in K14-mOVA mice. J Immunol. 2009 Apr 15;182(8):4665-74.
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Nervous System
Azim E, Shnider SJ, Cederquist GY, Sohur US, Macklis JD. Lmo4 and Clim1 Progressively Delineate Cortical Projection Neuron Subtypes during Development. Cereb Cortex. 2009 Apr 14. [Epub ahead of print]
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Qiu J, Takagi Y, Harada J, Topalkara K, Wang Y, Sims JR, Zheng G, Huang P, Ling Y, Scadden DT, Moskowitz MA, Cheng T. p27Kip1 constrains proliferation of neural progenitor cells in adult brain under homeostatic and ischemic conditions. Stem Cells. 2009 Apr;27(4):920-7.
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Stanco A, Szekeres C, Patel N, Rao S, Campbell K, Kreidberg JA, Polleux F, Anton ES. Netrin-1-alpha3beta1 integrin interactions regulate the migration of interneurons through the cortical marginal zone. Proc Natl Acad Sci USA. 2009 May 5;106(18):7595-600. Epub 2009 Apr 21.
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Pain
Scholz J, Mannion RJ, Hord DE, Griffin RS, Rawal B, Zheng H, Scoffings D, Phillips A, Guo J, Laing RJ, Abdi S, Decosterd I, Woolf CJ. A novel tool for the assessment of pain: validation in low back pain. PLoS Med. 2009 Apr 7;6(4):e1000047. Epub 2009 Apr 7.
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Renal System
Hopfer U, Hopfer H, Meyer-Schwesinger C, Loeffler I, Fukai N, Olsen BR, Stahl RA, Wolf G. Lack of Type VIII Collagen in Mice Ameliorates Diabetic Nephropathy. Diabetes. 2009 Apr 28. [Epub ahead of print]
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Sieber M, Hoffmann D, Adler M, Vaidya VS, Clement M, Bonventre JV, Zidek N, Rached E, Amberg A, Callanan JJ, Dekant W, Mally A. Comparative analysis of novel noninvasive renal biomarkers and metabonomic changes in a rat model of gentamicin nephrotoxicity. Toxicol Sci. 2009 Jun;109(2):336-49. Epub 2009 Apr 6. PubMed
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Takakura A, Contrino L, Zhou X, Bonventre JV, Sun Y, Humphreys BD, Zhou J. Renal Injury is a Third Hit Promoting Rapid Development of Adult Polycystic Kidney Disease. Hum Mol Genet. 2009 Apr 2. [Epub ahead of print]
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Vaidya VS, Ford GM, Waikar SS, Wang Y, Clement MB, Ramirez V, Glaab WE, Troth SP, Sistare FD, Prozialeck WC, Edwards JR, Bobadilla NA, Mefferd SC, Bonventre JV. A rapid urine test for early detection of kidney injury. Kidney Int. 2009 Apr 22. [Epub ahead of print]
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