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Research Newsletter - August 2008

Spotlight

This month's spotlighted article, published in the journal Cell, is by HSCI Executive Committe member Amy Wagers, PhD of the Joslin Diabetes Center.

Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles.

Abstract:

Satellite cells reside beneath the basal lamina of skeletal muscle fibers and include cells that act as precursors for muscle growth and repair. Although they share a common anatomical localization and typically are considered a homogeneous population, satellite cells actually exhibit substantial heterogeneity. We used cell-surface marker expression to purify from the satellite cell pool a distinct population of skeletal muscle precursors (SMPs) that function as muscle stem cells. When engrafted into muscle of dystrophin-deficient mdx mice, purified SMPs contributed to up to 94% of myofibers, restoring dystrophin expression and significantly improving muscle histology and contractile function. Transplanted SMPs also entered the satellite cell compartment, renewing the endogenous stem cell pool and participating in subsequent rounds of injury repair. Together, these studies indicate the presence in adult skeletal muscle of prospectively isolatable muscle-forming stem cells and directly demonstrate the efficacy of myogenic stem cell transplant for treating muscle degenerative disease.

Cerletti M, Jurga S, Witczak CA, Hirshman MF, Shadrach JL, Goodyear LJ, Wagers AJ. Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles. Cell. 2008 Jul 11;134(1):37-47. Read Paper.

Scientific Papers

Review and Commentary

  • Bonventre JV. Dialysis in acute kidney injury--more is not better. N Engl J Med. 2008 Jul 3;359(1):82-4. Epub 2008 May 20. Read Abstract.
  • Egli D, Birkhoff G, Eggan K. Mediators of reprogramming: transcription factors and transitions through mitosis. Nat Rev Mol Cell Biol. 2008 Jul;9(7):505-16. Review. Read Abstract.
  • Fenno LE, Ptaszek LM, Cowan CA. Human embryonic stem cells: emerging technologies and practical applications. Curr Opin Genet Dev. 2008 Jul 30. Read Abstract.
  • M√ɬºller LU, Schore RJ, Zheng Y, Thomas EK, Kim MO, Cancelas JA, Gu Y, Williams DA. Rac guanosine triphosphatases represent a potential target in AML. Leukemia. 2008 Jul 31. Read Abstract.
  • Raaijmakers MH, Scadden DT. Evolving concepts on the microenvironmental niche for hematopoietic stem cells. Curr Opin Hematol. 2008 Jul;15(4):301-6. Review. Read Abstract.
  • Tabin CJ, McMahon AP. Developmental biology. Grasping limb patterning. Science. 2008 Jul 18;321(5887):350-2. No abstract available. Read Abstract.
  • Williams DA. Progress reported in two studies of clinical gene transfer into the retina. Mol Ther. 2008 Jul;16(7):1181. Read Abstract.
  • Woolf CJ. Novel analgesic development: from target to patient or patient to target? Curr Opin Investig Drugs. 2008 Jul;9(7):694-5. No abstract available. Read Abstract.
  • Wu SM. Mesp1 at the heart of mesoderm lineage specification. Cell Stem Cell. 2008 Jul 3;3(1):1-2. Read Abstract.
  • Zhao R, Daley GQ. From fibroblasts to iPS cells: Induced pluripotency by defined factors. J Cell Biochem. 2008 Jul 30. Read Abstract.

Blood

  • Kindler T, Cornejo MG, Scholl C, Liu J, Leeman DS, Haydu JE, Frohling S, Lee BH, Gilliland DG. K-RasG12D-induced T-cell lymphoblastic lymphoma/leukemias harbor Notch1 mutations and are sensitive to {gamma}-secretase inhibitors. Blood. 2008 Jul 28. Read Abstract.
  • Lettre G, Sankaran VG, Bezerra MA, Ara√ɬ∫jo AS, Uda M, Sanna S, Cao A, Schlessinger D, Costa FF, Hirschhorn JN, Orkin SH. DNA polymorphisms at the BCL11A, HBS1L-MYB, and {beta}-globin loci associate with fetal hemoglobin levels and pain crises in sickle cell disease. Proc Natl Acad Sci USA. 2008 Jul 30. Read Abstract.

Cancer

  • De Keersmaecker K, Rocnik JL, Bernad R, Lee BH, Leeman D, Gielen O, Verachtert H, Folens C, Munck S, Marynen P, Fornerod M, Gilliland DG, Cools J. Kinase activation and transformation by NUP214-ABL1 is dependent on the context of the nuclear pore. Mol Cell. 2008 Jul 11;31(1):134-42. Read Abstract.
  • Hayakawa F, Abe A, Kitabayashi I, Pandolfi PP, Naoe T. Acetylation of PML is involved in histone deacetylase Inhibitor-mediated apoptosis. J Biol Chem. 2008 Jul 11. Read Abstract.
  • Huang G, Eisenberg R, Yan M, Monti S, Lawrence E, Fu P, Walbroehl J, L√ɬ∂wenberg E, Golub T, Merchan J, Tenen DG, Markowitz SD, Halmos B. 15-Hydroxyprostaglandin dehydrogenase is a target of hepatocyte nuclear factor 3beta and a tumor suppressor in lung cancer. Cancer Res. 2008 Jul 1;68(13):5040-8. Read Abstract.
  • Kuhnert F, Tam BY, Sennino B, Gray JT, Yuan J, Jocson A, Nayak NR, Mulligan RC, McDonald DM, Kuo CJ. Soluble receptor-mediated selective inhibition of VEGFR and PDGFRbeta signaling during physiologic and tumor angiogenesis. Proc Natl Acad Sci USA. 2008 Jul 22;105(29):10185-90. Epub 2008 Jul 16. Read Abstract.
  • Poliseno L, Pitto L, Simili M, Mariani L, Riccardi L, Ciucci A, Rizzo M, Evangelista M, Mercatanti A, Pandolfi PP, Rainaldi G. The proto-oncogene LRF is under post-transcriptional control of MiR-20a: implications for senescence. PLoS ONE. 2008 Jul 2;3(7):e2542. Read Abstract.
  • Shimamura T, Li D, Ji H, Haringsma HJ, Liniker E, Borgman CL, Lowell AM, Minami Y, McNamara K, Perera SA, Zaghlul S, Thomas RK, Greulich H, Kobayashi S, Chirieac LR, Padera RF, Kubo S, Takahashi M, Tenen DG, Meyerson M, Wong KK, Shapiro GI. Hsp90 inhibition suppresses mutant EGFR-T790M signaling and overcomes kinase inhibitor resistance. Cancer Res. 2008 Jul 15;68(14):5827-38. Read Abstract.
  • Upadhyay G, Goessling W, North TE, Xavier R, Zon LI, Yajnik V. Molecular association between beta-catenin degradation complex and Rac guanine exchange factor DOCK4 is essential for Wnt/beta-catenin signaling. Oncogene. 2008 Jul 21. Read Abstract.

Cardiovascular System

  • Antoniades C, Shirodaria C, Van Assche T, Cunnington C, Tegeder I, L√ɬ∂tsch J, Guzik TJ, Leeson P, Diesch J, Tousoulis D, Stefanadis C, Costigan M, Woolf CJ, Alp NJ, Channon KM. GCH1 haplotype determines vascular and plasma biopterin availability in coronary artery disease effects on vascular superoxide production and endothelial function. J Am Coll Cardiol. 2008 Jul 8;52(2):158-65. Read Abstract.

Developmental Biology

  • Beuling E, Bosse T, Aan de Kerk DJ, Piaseckyj CM, Fujiwara Y, Katz SG, Orkin SH, Grand RJ, Krasinski SD. GATA4 mediates gene repression in the mature mouse small intestine through interactions with Friend of GATA (FOG) cofactors. Dev Biol. 2008 Jul 26. Read Abstract.
  • Eminli S, Utikal JS, Arnold K, Jaenisch R, Hochedlinger K. Reprogramming of Neural Progenitor Cells into iPS Cells in the Absence of Exogenous Sox2 Expression. Stem Cells. 2008 Jul 17. Read Abstract.
  • McBratney-Owen B, Iseki S, Bamforth SD, Olsen BR, Morriss-Kay GM. Development and tissue origins of the mammalian cranial base. Dev Biol. 2008 Jul 22. Read Abstract.
  • Meissner A, Mikkelsen TS, Gu H, Wernig M, Hanna J, Sivachenko A, Zhang X, Bernstein BE, Nusbaum C, Jaffe DB, Gnirke A, Jaenisch R, Lander ES. Genome-scale DNA methylation maps of pluripotent and differentiated cells. Nature. 2008 Aug 7;454(7205):766-70. Epub 2008 Jul 6. Read Abstract.
  • Roesch K, Jadhav AP, Trimarchi JM, Stadler MB, Roska B, Sun BB, Cepko CL. The transcriptome of retinal M√ɬºller glial cells. J Comp Neurol. 2008 Jul 10;509(2):225-38. Read Abstract.

Diabetes

  • B√ɬ∂ni-Schnetzler M, Thorne J, Parnaud G, Marselli L, Ehses JA, Kerr-Conte J, Pattou F, Halban PA, Weir GC, Donath MY. Increased IL-1{beta} mRNA expression in {beta}-cells of individuals with type 2 diabetes and regulation of IL-1{beta} in human islets by glucose and auto-stimulation. J Clin Endocrinol Metabv. 2008 Jul 29. Read Abstract.

Genetics and Genomics

  • Dobyns WB, Mirzaa G, Christian SL, Petras K, Roseberry J, Clark GD, Curry CJ, McDonald-McGinn D, Medne L, Zackai E, Parsons J, Zand DJ, Hisama FM, Walsh CA, Leventer RJ, Martin CL, Gajecka M, Shaffer LG. Consistent chromosome abnormalities identify novel polymicrogyria loci in 1p36.3, 2p16.1-p23.1, 4q21.21-q22.1, 6q26-q27, and 21q2. Am J Med Genet A. 2008 Jul 1;146A(13):1637-54. Read Abstract.
  • Kantarci S, Ragge NK, Thomas NS, Robinson DO, Noonan KM, Russell MK, Donnai D, Raymond FL, Walsh CA, Donahoe PK, Pober BR. Donnai-Barrow syndrome (DBS/FOAR) in a child with a homozygous LRP2 mutation due to complete chromosome 2 paternal isodisomy. Am J Med Genet A. 2008 Jul 15;146A(14):1842-7. Read Abstract.
  • Morrow EM, Yoo SY, Flavell SW, Kim TK, Lin Y, Hill RS, Mukaddes NM, Balkhy S, Gascon G, Hashmi A, Al-Saad S, Ware J, Joseph RM, Greenblatt R, Gleason D, Ertelt JA, Apse KA, Bodell A, Partlow JN, Barry B, Yao H, Markianos K, Ferland RJ, Greenberg ME, Walsh CA. Identifying autism loci and genes by tracing recent shared ancestry. Science. 2008 Jul 11;321(5886):218-23. Read Abstract.

Imaging

  • Gee MS, Upadhyay R, Bergquist H, Alencar H, Reynolds F, Maricevich M, Weissleder R, Josephson L, Mahmood U. Human Breast Cancer Tumor Models: Molecular Imaging of Drug Susceptibility and Dosing during HER2/neu-targeted Therapy. Radiology. 2008 Jul 22. Read Abstract.
  • Hilderbrand SA, Kelly KA, Niedre M, Weissleder R. Near Infrared Fluorescence-Based Bacteriophage Particles for Ratiometric pH Imaging. Bioconjug Chem. 2008 Jul 31. Read Abstract.
  • Lin KY, Maricevich M, Bardeesy N, Weissleder R, Mahmood U. In vivo quantitative microvasculature phenotype imaging of healthy and malignant tissues using a fiber-optic confocal laser microprobe. Transl Oncol. 2008 Jul;1(2):84-94. Read Abstract.
  • Pfeffer CP, Olsen BR, Ganikhanov F, L√ɬ©gar√ɬ© F. Multimodal nonlinear optical imaging of collagen arrays. J Struct Biol. 2008 Jul 11. Read Abstract.
  • Taktak S, Weissleder R, Josephson L. Electrode chemistry yields a nanoparticle-based NMR sensor for calcium. Langmuir. 2008 Jul 15;24(14):7596-8. Epub 2008 Jun 18. Read Abstract.

Immunology

  • Johnsen AK, Plenge RM, Butty V, Campbell C, Dieguez-Gonzalez R, Gomez-Reino JJ, Shadick N, Weinblatt M, Gonzalez A, Gregersen PK, Benoist C, Mathis D. A broad analysis of IL1 polymorphism and rheumatoid arthritis. Arthritis Rheum. 2008 Jul;58(7):1947-57. Read Abstract.
  • Kovalovsky D, Uche OU, Eladad S, Hobbs RM, Yi W, Alonzo E, Chua K, Eidson M, Kim HJ, Im JS, Pandolfi PP, Sant'angelo DB. The BTB-zinc finger transcriptional regulator PLZF controls the development of invariant natural killer T cell effector functions. Nat Immunol. 2008 Jul 27. Read Abstract.

Muscular System

  • Cerletti M, Jurga S, Witczak CA, Hirshman MF, Shadrach JL, Goodyear LJ, Wagers AJ. Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles. Cell. 2008 Jul 11;134(1):37-47. Read Abstract.

Nervous System

  • Dimos JT, Rodolfa KT, Niakan KK, Weisenthal LM, Mitsumoto H, Chung W, Croft GF, Saphier G, Leibel R, Goland R, Wichterle H, Henderson CE, Eggan K. Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons. Science. 2008 Jul 31. Read Abstract.
  • Kim DS, Matsuda T, Cepko CL. A core paired-type and POU homeodomain-containing transcription factor program drives retinal bipolar cell gene expression. J Neurosci. 2008 Jul 30;28(31):7748-64. Read Abstract.
  • Sanchez-Pernaute R, Lee H, Patterson M, Reske-Nielsen C, Yoshizaki T, Sonntag KC, Studer L, Isacson O. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008 Aug;131(Pt 8):2127-39. Epub 2008 Jul 22. Read Abstract.
  • Seo H, Kim W, Isacson O. Compensatory changes in the ubiquitin-proteasome system, BDNF and mitochondrial complex II / III in YAC72 and R6/2 transgenic mice partially model Huntington's disease (HD) patients. Hum Mol Genet. 2008 Jul 17. Read Abstract.
  • Sepp KJ, Hong P, Lizarraga SB, Liu JS, Mejia LA, Walsh CA, Perrimon N. Identification of neural outgrowth genes using genome-wide RNAi. PLoS Genet. 2008 Jul 4;4(7):e1000111. Read Abstract.

Renal System

  • Kolpakova-Hart E, Nicolae C, Zhou J, Olsen BR. Col2-Cre recombinase is co-expressed with endogenous type II collagen in embryonic renal epithelium and drives development of polycystic kidney disease following inactivation of ciliary genes. Matrix Biol. 2008 Jul;27(6):505-12. Epub 2008 May 27. Read Abstract.

Skeletal System

  • Kolpakova-Hart E, McBratney-Owen B, Hou B, Fukai N, Nicolae C, Zhou J, Olsen BR. Growth of cranial synchondroses and sutures requires polycystin-1. Dev Biol. 2008 Jul 9. Read Abstract.

Skin

  • Tian T, Liu L, Freyschmidt EJ, Murphy GF, Kupper TS, Fuhlbrigge RC. Overexpression of IL-1alpha in Skin Differentially Modulates the Immune Response to Scarification with Vaccinia Virus. J Invest Dermatol. 2008 Jul 10. Read Abstract.

Technology

  • Blois J, Yuan H, Smith A, Pacold ME, Weissleder R, Cantley LC, Josephson L. Slow Self-Activation Enhances The Potency of Viridin Prodrugs. J Med Chem. 2008 Aug 14;51(15):4699-4707. Epub 2008 Jul 17. Read Abstract.
  • Breault DT, Min IM, Carlone DL, Farilla LG, Ambruzs DM, Henderson DE, Algra S, Montgomery RK, Wagers AJ, Hole N. Generation of mTert-GFP mice as a model to identify and study tissue progenitor cells. Proc Natl Acad Sci USA. 2008 Jul 29;105(30):10420-5. Epub 2008 Jul 23. Read Abstract.
  • Lee H, Sun E, Ham D, Weissleder R. Chip-NMR biosensor for detection and molecular analysis of cells. Nat Med. 2008 Aug;14(8):869-74. Epub 2008 Jul 6. Read Abstract.
  • Zhou Z, Koglin A, Wang Y, McMahon AP, Walsh CT. An eight residue fragment of an acyl carrier protein suffices for post-translational introduction of fluorescent pantetheinyl arms in protein modification in vitro and in vivo. J Am Chem Soc. 2008 Jul 30;130(30):9925-30. Epub 2008 Jul 2. Read Abstract.

Translational Medicine

  • Carraro A, Hsu WM, Kulig KM, Cheung WS, Miller ML, Weinberg EJ, Swart EF, Kaazempur-Mofrad M, Borenstein JT, Vacanti JP, Neville C. In vitro analysis of a hepatic device with intrinsic microvascular-based channels. Biomed Microdevices. 2008 Jul 5. Read Abstract.